Fig. 4: Cold exposure increases miR-378a-3p expression in BDEVs. | Nature Communications

Fig. 4: Cold exposure increases miR-378a-3p expression in BDEVs.

From: Cold-activated brown fat-derived extracellular vesicle-miR-378a-3p stimulates hepatic gluconeogenesis in male mice

Fig. 4: Cold exposure increases miR-378a-3p expression in BDEVs.The alternative text for this image may have been generated using AI.

a Venn diagram depicting the number fat depot-specific miRNAs within the top 15 expressed miRNAs in each indicated fat depot (n = 6 each group, mixed samples). b, c qPCR analysis of the relative levels of miR-378a-3p b and miR-193b-3p c (n = 8 each group, from 2 independent experiments). d Heatmap showing Z scores of miRNA expression in iBAT of mice placed at RT or cold for 72 h (GSE 4130635, n = 4 per group). e–i Eight-week-old male C57BL6/J mice were placed in RT or cold for 72 hours. e–g qPCR analysis of the levels of miR-378a-3p and miR-193b-3p in iBAT (e, n = 9 each group, from 2 independent experiments); in the equal amounts of RT-BDEVs or Cold-BDEVs (f, n = 6 each group, from 2 independent experiments) and serum-EVs from the same volume of serum (g, n = 8 each group, from 2 independent experiments). h qPCR analysis of the relative level of miR-378a-3p in liver (n = 8 each group, from 2 independent experiments). i qPCR analysis of the relative level of primary miR-378a-3p in iBAT and liver (n = 8 each group, from 2 independent experiments). j Eight-week-old male C57BL6/J mice treated with BDEVs each day for 3 injections (left panel). qPCR analysis of the absolute level of miR-378a in the liver 12 h post last injection. (n = 8 each group, from 2 independent experiments). k–m Cold-exposed eight-week-old male C57BL6/J mice were injected in situ with either GW4869 or control vehicle. Schematic diagram k; qPCR analysis of the relative miR-378a-3p level l and primary miR-378a-3p level m in liver. (n = 8 each group, from 2 independent experiments) n–p Sham-operated mice or mice with BATectomy were cold-exposed for 72 h. Experimental schematic n; qPCR analysis of the relative miR-378a-3p levels in the serum-EVs and liver o and primary miR-378a-3p in liver p (n = 8 each group, from 2 independent group). Mice were fasted for 4 h before sacrifice. Data presented as mean ± sem. Statistical analysis was performed using Mann Whitney test (two tailed) (j, o left panel) and others performed two-sided unpaired t-test. Source data are provided as a Source Data file.

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