Fig. 1: C. glutamicum strains carrying fluorescently-tagged ribosomes show wt-like growth behaviour and ribosome-associated fluorescence. | Nature Communications

Fig. 1: C. glutamicum strains carrying fluorescently-tagged ribosomes show wt-like growth behaviour and ribosome-associated fluorescence.

From: Growth-rate dependency of ribosome abundance and translation elongation rate in Corynebacterium glutamicum differs from that in Escherichia coli

Fig. 1: C. glutamicum strains carrying fluorescently-tagged ribosomes show wt-like growth behaviour and ribosome-associated fluorescence.The alternative text for this image may have been generated using AI.

a Growth curve (solid lines) and specific fluorescence (dashed lines) of the wt (black) and SM34 [(bL19-EYFP uS2-PAmCherry), green] strains from a BioLector cultivation in BHI + GLU medium. Shown is the mean and standard deviation (n = 3 biologically independent cultures). b DNA and ribosome distribution of exponentially growing and stationary phase cells of C. glutamicum SM30 (bL19-EYFP) cultivated on BHI + GLU. Similar results were obtained for two independent experiments. The first pair of panels on the left are bright-field microscopy images. The three pairs of panels on the right are wide-field fluorescence microscopy images. The SYTOX Orange-stained nucleic acid is shown in red and the bL19-EYFP-labelled ribosomes are shown in green. Scale bar = 1 µm. Source data are provided as a Source data file.

Back to article page