Fig. 5: Determination of NanoEPIC scores of TILs from murine PD-1 immunotherapeutic model.

a Proportion of CD45 + CD8 + T cells arranged with increasing NanoEPIC scores from left to right. Flow cytometric analysis of proliferation markers ki67 b, T cell differentiation and activation markers (TCF7, CD69, and CD137) c–e, cytotoxicity (IFNγ, Granzyme B) f, g and immune-checkpoint markers (PD-1 and CD152) h, i shown as scatterplots of median fluorescence intensities and NanoEPIC score. All scatterplots were fitted with linear regression analysis. Source data are provided as a Source Data file.