Fig. 2: Subsequent WNT inhibition promotes PGCLC generation and represses mesodermal markers. | Nature Communications

Fig. 2: Subsequent WNT inhibition promotes PGCLC generation and represses mesodermal markers.

From: Monolayer platform to generate and purify primordial germ-like cells in vitro provides insights into human germline specification

Fig. 2

a Schematic of WNT agonism (CHIR) or WNT antagonism (XAV939) or no WNT manipulation (base media) at different timepoints during PGCLC monolayer differentiation. b FACS data showing efficiency of generating NANOG+ PGCLCs under different conditions of WNT pathway manipulation at Day 2.5 and Day 3.5. Data are presented as mean values ± SEM. Statistical test–two-way ANOVA, with Tukey multiple testing correction. n = 3 biological replicates/group for all except for CHIR99021 where n = 4 biological replicates/group. Adjusted P values are shown above error bars. Source data are provided as a Source Data file. c Log2 normalized expression levels of PGC and mesodermal markers in Base media vs. XAV939 vs. CHIR treated samples at different timepoints. n = 3 biological replicates/group.

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