Fig. 7: Engineering of tomato germplasm with high melatonin content. | Nature Communications

Fig. 7: Engineering of tomato germplasm with high melatonin content.

From: Understanding the mechanism of red light-induced melatonin biosynthesis facilitates the engineering of melatonin-enriched tomatoes

Fig. 7

a CRISPR/Cas9 target site design and sequencing results of gene editing for SlPIF4. b CRISPR/Cas9 target site design and sequencing results of gene editing for the promoter of SlCOMT2. Cas9 recognize NG-PAM sequence. c Melatonin content of WT and T2 CR fruits at the Br+3 stage. Data is represented as Mean ± SEM (n = 3). 10–12 tomato fruits from the same seedling were pooled as one biological replicate. The P values indicate the results from pairwise comparisons of one-way ANOVA tests. Different letters represent a significant difference at P < 0.05. Fruit phenotype of WT and CR fruits at the Br+3 and the Br+7 stage were also presented. Source data are provided as a Source Data file.

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