Fig. 7: InterTAD-level insulation is increased by cohesin loss.

a Relative enrichment of the interaction frequency (log scale) relative to control. The region and TAD labels (A1–A5, B1 and B2) are as in Fig. 4a. See Fig. S9a for all samples. b Top: Schematic illustration of DRF. Middle: Heatmap of DRF for depletions (row) for the same region as shown in (a). Bottom: Average DRF of cohesin and loaders (red), unloaders (blue), and others (black). The shaded regions indicate the 99% confidence interval. The purple lines at the bottom of the graph indicate the identified differential DRF regions. c Examples of differential DRF regions. Top: Normalized Hi-C matrix (control) and relative interaction frequency after depletions. The dashed black triangles indicate TADs in the control sample. Bottom: Compartmental PC1 values and ChIP-seq distribution. d Compartmental PC1 values for the control and siNIPBL samples centered on 241 differential DRF regions. Rows were ordered using hierarchical clustering based on the control sample. e The cosine similarity distribution of the relative interaction frequency between each Hi-C sample pair for all 241 differential DRF regions (~2 Mbp from the center of each region). Whisker plots with median and IQR. See Fig. S9b for all comparisons. Source data are provided as a Source Data file.