Fig. 4: NET4 proteins interact with RabG3 GTPases in an activation- and motif-dependent manner. | Nature Communications

Fig. 4: NET4 proteins interact with RabG3 GTPases in an activation- and motif-dependent manner.

From: NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity

Fig. 4

a Yeast-two-hybrid analysis of NET4A and NET4B indicate interactions with constitutive active (CA = Q67L) but not dominant-negative (DN = T22N) RABG3 members. b Yeast-two-hybrid analysis of NET4A and NET4B shows no interactions with members of other Rab GTPase families than RABG3. c Sequence alignment of IRQ domains including the C-terminus (CT). Of all NET family members, IRQ domains are only found in NET4A, NET4B, NET3A and NET3C. d Overview of truncated NET4B proteins used to identify the minimal interaction domain required for interaction with RABG3 proteins. e, f Yeast-two-hybrid analysis of the indicated truncated NET4B proteins with constitutive active (CA = Q67L) RABG3a; NAB = NET actin binding domain; IRQ = conserved sequence motif; CT = C-terminus. All experiments were repeated at least twice with similar results.

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