Fig. 7: Guard cell actin dynamics are altered in net4 mutants.

a, b, d, e, g, h Quantification of actin cytoskeletal patterns of Lifeact-mNeonGreen-expressing Col-0 WT and net4ab-c2 plants in guard cells. ImageJ analysis using the LPIXEL Inc LPX plugin set. a, b Mean angular difference (a) and parallelness (b) of the actin cytoskeleton in untreated guard cells. d, e Mean angular difference (d) and parallelness (f) of the actin cytoskeleton in guard cells treated for 1 h with flg22. g, h Mean angular difference (g) and parallelness (h) of the actin cytoskeleton in guard cells treated for 2 h with flg22. Box plots of the values are shown with whiskers from the 5th to 95th percentiles, the line in the box shows the median; 1 h flg22 treatment: WT Col-0 n = 73, net4ab-c2 n = 64 guard cells, p < 0.0001 Mann Whitney t-test; 2 h flg22 treatment: WT Col-0 n = 38, net4ab-c2 n = 23 guard cells, p = 0.0777 ns Mann Whitney t-test; Stomata opening buffer: WT Col-0 n = 10, net4ab-c2 n = 7 guard cells, - angle p = 0.3638, parallelness p = 0.1088 ns Mann Whitney t-test. c, f, i Representative super-resolution Airyscan confocal images of Lifeact-mNeonGreen-positive actin filaments in Col-0 WT and net4ab-c2 untreated and treated for 1 and 2 hrs with flg22, respectively. Scale bars = 10 µm. The experiments were repeated at least thrice with similar results.