Fig. 5: The production of poly-GR from AUG-translation is low. | Nature Communications

Fig. 5: The production of poly-GR from AUG-translation is low.

From: Single-molecule imaging reveals distinct elongation and frameshifting dynamics between frames of expanded RNA repeats in C9ORF72-ALS/FTD

Fig. 5: The production of poly-GR from AUG-translation is low.

a Diagram of DPR elongation luciferase reporters. The DPR of interest is in frame with N-terminal AUG-HiBiT-APEX2 and C-terminal Flag tag. b HiBiT blotting (top) or western blotting with Flag antibody (bottom) to measure the DPR products with N- and C-terminal tags. c Densitometry quantification of the relative reporter expressions by HiBiT blot. Data are presented as mean ± SEM from three (GA, GR) or one (GP) independent experiments. d Densitometry quantification of the relative reporter expressions by Flag blot. Data are presented as mean ± SEM from three (GA, GR) or one (GP) independent experiments.

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