Fig. 6: Progenitor-progeny relationship of PD-1⁺ CD8⁺ T cell subsets in acute GvHD.

A Pseudotime analysis of PD-1⁺ alloreactive CD8⁺ T cells showing a differentiation trajectory where cells begin as progenitor cells (Prog_T), transition through proliferating cells (Prolif_T), and finally become terminally differentiated cells (Term_T). B, C PD-1⁺CD39^- cells containing a large fraction of TCF1⁺ cells and PD-1⁺CD39⁺ cells, mostly TCF1^-, were isolated from the spleen of GvHD B6 recipients of Balb/c T cells at 7 dpt and co-cultured with naïve CD45.1⁺ B6 splenocytes in the presence of CD8⁺-depleted naïve Balb/c splenocytes for 5 days. B, C Phenotypic analysis (B) and summary graph (C) showing CD39⁺ and CD39^- proportions among sorted PD-1⁺CD39^- and PD-1⁺CD39⁺ CD8⁺ T cell subsets before and after the MLR. Data are representative of two similar experiments, n = 3 per experiment. Source data are provided as a Source Data file.