Fig. 7: In vivo exclusive proliferative potential of the TCF1+ CD8+ T cell subset and their differentiation into Tim-3+ cells upon acute GvHD. | Nature Communications

Fig. 7: In vivo exclusive proliferative potential of the TCF1+ CD8+ T cell subset and their differentiation into Tim-3+ cells upon acute GvHD.

From: Defining a TCF1-expressing progenitor allogeneic CD8+ T cell subset in acute graft-versus-host disease

Fig. 7

A Experimental setup (1): PD-1+Tim-3+ and PD-1+Tim-3- CD8+ T cells were isolated from the spleen of GvHD Balb/c recipients of B6 T cells at 7 dpt. B Population of TCF1+ cells among sorted PD-1+ CD8+ T cells. C Experimental setup (2): Sorted CD8+ T cells (2 × 105) were transferred into lethally irradiated Balb/c mice with 1 × 107 bone marrow cells intravenously. D, E Representative flow plots (D) and summary graphs (E, top) showing the frequency of sorted CD45.2+ cells among CD8+ T cells and their absolute number (E, bottom) in the indicated tissues. A dashed line indicates the number of transferred cells, assuming a 10% engraftment rate. F, G Phenotypic analysis (F) and summary graph (G) showing the proportions of Tim-3+ and Tim-3- within sorted CD8+ T cell subsets in the spleen at 7 dpt compared to the pre-transplantation state. Data were obtained from a single experiment (n = 5 for PD-1+Tim-3+ cells and n = 4 for PD-1+Tim-3- cells), and the mean and SEM were shown. Statistical significance was determined by a two-tailed unpaired t-test. Source data are provided as a Source Data file.

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