Fig. 8: Impaired proliferative potential of PD-1+ alloreactive CD8+ T cells in acute GvHD.
A Experimental setup: (1) Naïve Balb/c splenocytes (2 × 105), (2) GvHD Balb/c-derived splenocytes (2 × 105), and (3) GvHD Balb/c-derived CD8+ T cells (4 × 104) plus CD8+-depleted naïve Balb/c splenocytes (1.6 × 105) were co-cultured with naïve CD45.1+ B6 splenocytes (2 × 105) for 5 days in two-way MLR. Splenocytes of GvHD B6 recipients of Balb/c T cells were isolated at 21 dpt. B, C Representative plots (B) and summary graph (C) showing the proportion of CFSE-diluted cells among CD8+ T cells in the indicated conditions. Data were obtained from a single experiment (n = 3 of technical replicates), and the mean and SEM were shown. Statistical significance was determined by one-way ANOVA with post hoc Tukey’s multiple comparisons test and was only shown for comparison within each mouse strain or between the same groups. D Representative plots of the proportion of CD8+ T cells at each division among Balb/c-derived CD8+ T cells. E Summary graph showing the proportion of CD8+ T cells at each division among CFSE-diluted Balb/c-derived CD8+ T cells. Statistical significance was determined by one-way ANOVA with post hoc Tukey’s multiple comparisons test. *p < 0.05 and **p < 0.01 from the comparison with the group stimulated with naïve Balb/c splenocytes (1, red); †p < 0.05 and ††p < 0.01 from the comparison with the group stimulated with GvHD Balb/c-derived splenocytes (2, blue). Source data are provided as a Source Data file.
