Fig. 9: Ex vivo IL-15-driven proliferation of the PD-1+ alloreactive CD8+ T cell subsets in acute GvHD. | Nature Communications

Fig. 9: Ex vivo IL-15-driven proliferation of the PD-1+ alloreactive CD8+ T cell subsets in acute GvHD.

From: Defining a TCF1-expressing progenitor allogeneic CD8+ T cell subset in acute graft-versus-host disease

Fig. 9

A, B Representative flow plots (A) and summary graphs (B) showing the expression of CD122 (IL-2/15Rβ) and CD132 (common ɣ chain receptor) on the indicated CD8+ T cell subsets at 28 dpt. Data are representative of two independent experiments (n = 4/experiment). Statistical significance was determined by one-way ANOVA with post hoc Tukey’s multiple comparisons test. C, D CD8+ T cells isolated from the spleen of GvHD B6 recipients of Balb/c T cells were labeled with CFSE and then cultured in the presence or absence of 100 ng/ml recombinant mouse IL-15 protein in addition to naïve Balb/c splenocytes for 3 days. C Gating strategy to determine TCF1+CD39- and TCF1- CD39+ subsets and their proliferation three days after the incubation. D Summary graph showing the proportion of CFSE-diluted cells among the indicated CD8+ T cell subsets. Data are representative of two similar experiments (n = 4 or 5/experiment). Statistical significance was determined by a two-tailed paired t-test. Source data are provided as a Source Data file.

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