Fig. 2: Regulation of NAD+ salvage associated genes ex and in vivo.
From: NAD+ metabolism is a key modulator of bacterial respiratory epithelial infections
A–C Primary human bronchial epithelial cells from healthy donors were differentiated into pseudostratified respiratory epithelium as air-liquid interface cultures. After differentiation, cells were apically infected with Spn D39, MOI 20 for 16 h. The expression of NAMPT (B) and NMNAT1 (C) was determined by qPCR. D–F Human lung tissue explants were injected with Spn D39 or PBS and incubated for 12 h (D). Expression of NAMPT (E) and NMNAT1 (F) was determined by qPCR. G, H 3 published microarray transcriptome datasets of murine lungs infected with different strains of Spn D39 were analyzed for the expression of NAMPT (G) and NMNAT1 (H). Study 1: GSE83612, Mouse strain: C57BL/6, male and female; 24 h post-inoculation with 5 × 106 CFU, serotype: 19; Study 2: GSE45644, mouse strain: BALB/C, female; serotype 2, 48 h post-inoculation with 5 × 104 CFU; Study 3: GSE61459, mouse strain: BALB/C, female; serotype: 2, 24 h post-inoculation with 5 × 106 CFU. Statistics: two-tailed paired t-test (B, C, E, F); two-tailed unpaired t-test (G, H); n = 6 biologically distinct samples (B, C); 4 biologically distinct samples (E, F) 8 distinct animals (study 1), 9 distinct animals (study 3), 10 distinct animals (study 2) (G, H); box plots: line at mean, box ranges from min to max; results are normalized to untreated controls of the corresponding donor (C, D) or average expression values of control tissue/animals (E–H). Drawings were designed using GraphPad prism. MOI multiplicity of infection.
