Fig. 5: Transcriptional regulation of NMNAT1 is induced by pneumolysin (ply).
From: NAD+ metabolism is a key modulator of bacterial respiratory epithelial infections
A–C BEAS-2B cells were infected with Spn D39, WT or Δply for 16 h. Gene expression was determined by qPCR. D–F BEAS-2B cells were treated with pneumolysin in indicated concentrations for 16 h. Expression of IL-8, NAMPT and NMNAT1 was determined as indicated. G–I BEAS-2B cells were treated with 120 nM inactive pneumolysin for 16 h. Expression of IL-8, NAMPT and NMNAT1 was determined as indicated. Statistics: two-tailed paired t-test (A–C, G–I); sigmoidal dose-response fit with variable slope (D–F); Significances were determined against wild-type infected (A–C) or unstimulated controls; N = 4 biologically distinct samples (A–C) 3 biologically distinct samples (D–I); box plots: line at mean, box ranges from min to max; results are normalized against untreated controls. Ply pneumolysin.
