Fig. 5: Identity of IL-22 expressing cells and their localization to regions of BC hyperplasia in lungs of PR8 infected mice.

A Experimental design for fate mapping of IL-22 expressing cells following PR8 infection using IL-22^Cre/ROSA-tdT mice. (B) Flow cytometry analysis of either BAL or tissue homogenate from PR8-infected IL-22^Cre/ROSA-tdT mice. IL-22 lineage reporter (tdT; Lin⁺) was assessed as a function of surface expression of CD3 and CD4 and data was presented as the fraction of positive cells. Data are presented as mean values ±± SEM. n = 5 per naïve condition and n = 4 per PR8 condition. All analyzed samples were biologically independent. Significance was determined by Mann–Whitney two-tailed U-test (*P < 0.05). Source data are provided as a Source Data file. C Immunofluorescence detection of Lin⁺ cells as a function of p63-immunoreactive BC within BC-rich vs. BC devoid alveolar epithelium of PR8-infected mice (1 experiment, n = 5 biological replicates). D Immunofluorescence detection of γδTcr⁺ cells as a function of Krt5-immunoreactive BC within BC-rich vs. BC devoid alveolar epithelium of PR8-infected mice (1 experiment, n = 5 biological replicates). E Immunofluorescence localization of IL-22 expressing cells 7 and 14 days post-PR8 infection (1 experiment, n = 5 biological replicates). F Immunofluorescence colocalization of IL-22ra1 (red) with either a-tub (ciliated), Scgb3a2 (serous/club), Krt5 (BC), Scgb1a1 (club) (green) in airways of naïve mice (1 experiment, n = 5 biological replicates). G Representative immunofluorescent colocalization of IL-22ra1 (red) and Krt5 (green) in BC-rich alveolar region of PR8-infected mouse lung (1 experiment, n = 5 biological replicates).