Fig. 7: Tissue-resident memory (TRM) cells in the lungs of vaccinated mice. | Nature Communications

Fig. 7: Tissue-resident memory (TRM) cells in the lungs of vaccinated mice.

From: A Glycolipidated-liposomal peptide vaccine confers long-term mucosal protection against Streptococcus pyogenes via IL-17, macrophages and neutrophils

Fig. 7: Tissue-resident memory (TRM) cells in the lungs of vaccinated mice.The alternative text for this image may have been generated using AI.

BALB/c mice (n = 5 mice/group; female, 4–6 weeks old) were immunized i.n. on days 0, 21, and 42 with PBS, J8-Lipo-DT-PHAD, J8-Lipo-DT, Lipo-DT, Lipo-DT-PHAD, J8-Lipo-PHAD, J8-Lipo, Lipo-PHAD or Lipo. Six weeks post-last-immunization mice were euthanized, and lungs excised. Data represented as the frequency of TRM (CD103−CD69+) cells from effector memory (EM; CD62L−CD44+) CD4+ cells (boxplot–min/max; medium with SEM). Statistical analysis was performed using one-way ANOVA (*p < 0.05; **p < 0.01) for multiple comparisons (Dunnett’s multiple comparisons test). Samples were run on a BD LSRFortessa cytometer and data analyzed with FlowJo v10.8 software (BD Biosciences). The experiment was repeated once to confirm the results. Source data are provided as a Source Data file.

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