Fig. 2: The funiculi, integuments, and megagametogenesis were abnormal in the tcpDUO ovules. | Nature Communications

Fig. 2: The funiculi, integuments, and megagametogenesis were abnormal in the tcpDUO ovules.

From: Arabidopsis TCP4 transcription factor inhibits high temperature-induced homeotic conversion of ovules

Fig. 2: The funiculi, integuments, and megagametogenesis were abnormal in the tcpDUO ovules.The alternative text for this image may have been generated using AI.

a–d SEM analysis of the mature ovules from wild-type (a, b) and tcpDUO (c, d). b and c, Close-up view of ovules from a and b. The red arrows indicate the ovules with abnormal integuments. The cells of funiculi are marked with red lines in b and d to show the different number of cells consisting of funiculi in wild-type and tcpDUO. Images of a–d are representatives of at least five independent individuals. e–g Representative images of DIC micrographs of ovules from wild-type (e) and tcpDUO (f, g) ovules at the FG7 stage. Green-shaded areas are the nucellus in the wild-type ovule. Pink-shaded areas are inner integuments. Yellow-shaded areas are the outer integuments. The egg cell and central cell were clearly observed in the nucellus of wild-type plants (e). Excessive growth of outer or inner integuments was observed in the ovules of tcpDUO (f, g). h–k DIC micrographs of ovules from wild-type (h, j) and tcpDUO (i, k) during megasporogenesis. Images are representatives of at least five independent individuals. Source data for f–g are provided as a Source Data file. Pink-shaded areas are the megaspore mother cells. Green-shaded areas are the functional megaspore cells. MMC, megaspore mother cell. FM, functional megaspore. DM, degenerated megaspore. Mp, micropyle. Ch, chalaza. Ec, egg cell. Cc, central cell. Nc, nucellus. Ii, inner integument. Oi, outer integuments. Scale bars, 100 μm in a and c, 10 μm in b, d, h–k. 20 μm in e–g.

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