Fig. 1: Endo-d-arabinanases identified from Mi. arabinogalactanolyticum. | Nature Communications

Fig. 1: Endo-d-arabinanases identified from Mi. arabinogalactanolyticum.

From: Identification and characterization of endo-α-, exo-α-, and exo-β-d-arabinofuranosidases degrading lipoarabinomannan and arabinogalactan of mycobacteria

Fig. 1

a A structural model of LAM from My. tuberculosis (MtLAM). The d-arabinan domain consists mainly of α-links, but there are β-links on the non-reducing terminal side. Mannose residues cap the β-linked d-Araf in MtLAM. LM, lipomannan domain. b SDS-PAGE of purified native endo-d-arabinanase from Mi. arabinogalactanolyticum (nEndoMA) and stained by Coomassie brilliant blue. M, protein molecular weight marker. c, d Degradation of MtLAM analyzed using SDS-PAGE with silver-PAS staining (c) and HPAEC-PAD (d). MtLAM was incubated with each enzyme in 50 mM sodium phosphate (pH 6.0) at 37 °C overnight. A1-A20 in (d) indicates substrate-released arabinooligosaccharides. Numbers represent the degree of polymerization. e The d-arabinan degradation PUL of Mi. arabinogalactanolyticum.

Back to article page