Fig. 2: The cell shape index decreases with increasing monolayer density.
From: Hexanematic crossover in epithelial monolayers depends on cell adhesion and cell density
a, b Confocal image of confluent MDCK-II WT and E-cad KO monolayers (green, ZO-1 and blue, nuclei), cultured on non-coated glass. Cells were segmented, and their shape reconstructed by connecting the vertices. c Mean cell shape index per experimental image of both cell lines. The box shows the median (red line), 25th and 75th percentiles (box), maximum and minimum without outliers (whiskers), and 95% confidence interval of the median (notches). d Distribution of the mean cell-cell distance, Rcc. The bin-width is 0.2 μm. For further analysis, monolayers were grouped in six intervals, D1–D6, of 2.6 μm-width each. e Mean shape index increases with Rcc. Error bars represent the standard deviation. f The probability distribution of the number of nearest neighbors per cell. Statistics: c–f NWT = 226 and NKO = 216 from three independent experiments. c, e p-values are calculated from two-sided Wilcoxon rank sum test: nsp > 0.5. Source data are provided as Source Data file.
