Fig. 4: Transition zone and axoneme structure remain intact in iCre/Tctn1^flox outer segments.

a Representative TEM images from cross-sections of 3 month iCre/Tctn1^het and iCre/Tctn1^flox retinas. Low magnification to the left; high magnification to the right. Red arrowhead marks disorganized discs. Yellow asterisks indicate bulge in axoneme at distal end of transition zone. Scale bars, 500 nm. b Representative Airyscan images of isolated outer segments from iCre/Tctn1^het and iCre/Tctn1^flox retinas stained with α-tubulin (green) and WGA (gray). Scale bar, 2 µm. Averaged intensity plot is shown below. WGA intensity of 10–15 transition zones was analyzed for n = 3 biological replicates. Error bars indicate S.D. c Representative TEM images of the transition zone from iCre/Tctn1^het and iCre/Tctn1^flox retinal cross-sections. Yellow arrowheads indicate the presence of the ciliary necklace present at the membrane of iCre/Tctn1^het and iCre/Tctn1^flox outer segments. Scale bar, 200 nm. d Representative freeze fracture TEM images of the transition zone of iCre/Tctn1^flox outer segments. Red box outlines magnification of the transition zone region of iCre/Tctn1^flox outer segments to the right. Scale bars, 500 nm. Source data are provided as a Source Data file.