Fig. 1: Characterization of postnatal morphogenesis of dermal lymphatic vessel network. | Nature Communications

Fig. 1: Characterization of postnatal morphogenesis of dermal lymphatic vessel network.

From: Self-organized and directed branching results in optimal coverage in developing dermal lymphatic networks

Fig. 1: Characterization of postnatal morphogenesis of dermal lymphatic vessel network.The alternative text for this image may have been generated using AI.

A Mouse ear pinna ventral dermis stained for LYVE1 at postnatal day (P) 4, 6, 8, 13, 16, and 21. The dashed yellow line indicates the ear pinna boundary. The boxed regions are shown as magnified images. The white arrows indicate the LV trees that invade the dermis from the stalk of the ear pinna, whereas the yellow arrows indicate LV trees that invade the ventral dermis from the tip of the ear pinna. Scale bars are 1 mm in the overview and 100 μm in magnified images. See also Supplementary Movies 1–9. B Manual tracing of the lymphatic endothelial sub-trees growing on the superficial ventral dermis in P6 and P8 ear pinna. Each sub-tree is indicated with a unique color. Images represent n = 5 P6 and n = 5 P8 ear pinna, representing 5 mice each. See Supplementary Fig. 1F for additional samples and Supplementary Movies 2, 3, and 5. Scale bars are 500 μm. C Graph shows mean +/− SD of the mouse ear pinna area. Number of analyzed ear pinna for P1 n = 2 (in 1 mouse), P2 n = 2 (1 mouse), P4 n = 17 (9 mice), P5 n = 4 (2 mice), P6 n = 10 (5 mice), P7 n = 4 (2 mice), P8 n = 8 (4 mice), P9 = 2 (2 mice), P13 n = 10 (6 mice), P16 n = 17 (12 mice) and P21 n = 14 (13 mice). D–F Dot plots showing the mean +/− SD of D the segment number, E segment length, and F the total LYVE1-positive vessel length. Number of analyzed ventral ear pinna for P4 n = 16 (in 8 mice), P6 n = 8 (5 mice), P8 n = 4 (4 mice), P13 n = 6 (5 mice), P16 n = 6 (6 mice), and P21 n = 6 (6 mice). Source data for Fig. 1C–F are provided as a Source data file.

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