Fig. 2: Darapladib sensitises cells to ferroptosis induced by GPX4 inhibition or cysteine deprivation.
a Relative viability of Hs746T and SNU-484 cells treated with increasing concentrations of ML210 or JKE1674 in the presence or absence of 2 μM darapladib for 20 h. The data are presented as the means ± SDs (n = 3 or 4 independent experiments). b Relative viability of cells in the presence or absence of 1 μM Fer-1. The data are presented as the means ± SDs (n = 3 or 4 independent experiments with, the significance of the results was assessed using a two-tailed Student’s t test). c Viability of Hs746T and SNU-484 cells cultured in cysteine-deficient medium in the presence and absence of 2 μM darapladib for 18 h. The data are presented as the means ± SDs (n = 4 independent experiments with, the significance of the results was assessed using a two-tailed Student’s t test). d, e Lipid peroxidation levels in Hs746T and SNU-484 cells treated with 0.2 μM RSL3 and 2 μM darapladib for 1 h. Lipid oxidative potential was assessed by flow cytometry (d) and microscopy (e) using C11-BODIPY581/591. Probes fluorescing green represent those that have been oxidised. Experiments were repeated three times. Scale bar, 200 μm. d The data are presented as the means ± SDs (n = 3 independent experiments with, the significance of the results was assessed using a two-tailed Student’s t test). f Lipid peroxidation levels in Hs746T cells cultured in cysteine-deficient medium in the presence or absence of darapladib for 12–14 h. The data are presented as the means ± SDs (n = 3 independent experiments). Exact p values provided as source data. Source data are provided as a source data file.
