Fig. 7: ACOD1 deletion promoted the anti-ovarian cancer activity of iMACs combining with ICIs in vivo. | Nature Communications

Fig. 7: ACOD1 deletion promoted the anti-ovarian cancer activity of iMACs combining with ICIs in vivo.

From: Metabolic Reprogramming via ACOD1 depletion enhances function of human induced pluripotent stem cell-derived CAR-macrophages in solid tumors

Fig. 7: ACOD1 deletion promoted the anti-ovarian cancer activity of iMACs combining with ICIs in vivo.The alternative text for this image may have been generated using AI.

a A schematic of the in vivo study using HO-8910 cells for a mouse ovarian orthotopic injection model treated with MSLN-CAR-iMACs and ACOD1-/- MSLN-CAR-iMACs, and combined with an anti-CD47 antibody. b Tumor burden was determined by bioluminescent imaging (BLI). Images of representative time points were shown (n = 5 per group). c Quantification of tumor burden of representative time points was displayed as mean ± SD. Statistics by two-way ANOVA test. (PBS vs anti-CD47, P > 0.9999; PBS vs anti-CD47 + MSLN-CAR-iMAC, P = 0.004; PBS vs anti-CD47 + ACOD1-/- MSLN-CAR-iMAC, P < 0.0001; anti-CD47 + MSLN-CAR-iMAC vs anti-CD47 + ACOD1-/- MSLN-CAR-iMAC, P < 0.0001) d The Kaplan-Meier curve demonstrating survival of the mice. Statistics by two-tailed log-rank test. e A schematic of the in vivo study using HO-8910 cells for a mouse intraperitoneal (IP) injection model treated with MSLN-CAR-iMACs and ACOD1-/- MSLN-CAR-iMACs, and combined with an anti-PD1 antibody. f Tumor burden was determined by BLI. Images of representative time points were shown (n = 5 per group). g Quantification of tumor burden of representative time points was displayed as mean ± SD. Statistics by two-way ANOVA test. (PBS vs anti-PD1, P = 0.9078; PBS vs anti-PD1 + MSLN-CAR, P < 0.0001; PBS vs anti-PD1 + ACOD1-/- MSLN-CAR, P < 0.0001; anti-PD1 + MSLN-CAR vs anti-PD1 + ACOD1-/- MSLN-CAR, P < 0.0001) h The Kaplan-Meier curve demonstrating survival of the mice. Statistics by two-tailed log-rank test. Source data are provided as a Source Data file.

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