Fig. 3: RNA sequencing reveals the immune signature of CXCR4hi neutrophils.
From: CREB1-driven CXCR4hi neutrophils promote skin inflammation in mouse models and human patients
a Volcano plot showing the number of differentially expressed genes (DEGs) between CXCR4lo and CXCR4hi neutrophils from healthy controls (n = 7) and psoriasis patients (n = 6). b Heatmap of DEGs between psoriatic CXCR4lo and CXCR4hi neutrophils related to neutrophil and immune function. c Enriched GO terms between CXCR4lo and CXCR4hi psoriatic neutrophils. d Gene set enrichment analysis showing enrichment of genes involved in glycolysis in psoriatic CXCR4hi neutrophils. e Flow cytometry of glycolytic markers in CXCR4lo and CXCR4hi neutrophils from healthy controls and psoriasis patients. f Uptake of glucose (2-NBDG) in CXCR4lo and CXCR4hi neutrophils from healthy controls and psoriasis patients. g Extracellular lactate production in CXCR4lo and CXCR4hi neutrophils from healthy controls and psoriasis patients. Mean ± SD (n = 6 biologically independent samples/group). Two-way ANOVA with Tukey’s post hoc test was performed as two-sided analyses and adjusted for multiple comparisons in the statistical analyses. ns, not significant. HC, healthy control; MFI, mean fluorescence intensity; Pso, psoriasis patients; RNA-seq, RNA sequencing. Source data are provided as a Source Data file.
