Fig. 7: NPM covalent occupancy and disulfide crosslinking affect the inhibitory effect of quercetin on hP2X3.
From: Chronic cough relief by allosteric modulation of P2X3 without taste disturbance
a Chemical structure of NPM and its modification of cysteine at the mutation site. b Representative currents recorded from cells transfected with P2X3S67C receptors. Cells were voltage-clamped at −60 mV and currents were evoked by ATP (10 μM) at 7 min intervals. NPM (2 mM) and quercetin (100 μM) were applied as indicated. c Pooled data for experiments in b. The inhibition rates of quercetin before and after the NPM treatments for individual cells are connected with lines. Each line represents an independent cell, n = 5. P value was calculated from a paired, two-tailed t test. d Representative currents recorded from cells transfected with WT hP2X3 and its double cysteine mutants, D158C/E111C and L127C/T202C. ATP, H2O2, DTT, and quercetin were applied as indicated. e Pooled data for experiments in d. Each circle represents an independent cell, n = 3 for WT hP2X3, n = 5 for L127C/T202C (DTT treated), n = 4 for L127C/T202C (H2O2 treated), n = 4 for D158C/E111C (DTT treated), n = 5 for D158C/E111C (H2O2 treated). P value was calculated from an unpaired, two-tailed t test. All summary data are presented as mean ± SEM. Source data are provided as a Source Data file.
