Fig. 1: Tph1–/–mice are susceptible to A. fumigatus infection.
From: Bridging of host-microbiota tryptophan partitioning by the serotonin pathway in fungal pneumonia
Tph1+/+ and Tph1–/– littermates were infected intranasally with A. fumigatus. Mice were sacrificed at 3 or 7 days post infection (dpi) and monitored for a fungal growth (n = 6 mice/group), b number of neutrophils and monocytes (n = 10 fields over 6 mice/group) in the bronco-alveolar lavage, c, d lung inflammatory cytokine and chemokine expression (c, n = 2; d, n = 4–6 mice/group), e in vitro effector activity (n = 6 mice/group) and reactive oxygen species production of ex-vivo lung macrophages (DHE and DHR, n = 39 readings over six mice/group) and neutrophils (DHE, n = 13 and DHR, n = 28 readings over 6 mice/group) from uninfected mice, f lung histology (periodic acid-Schiff staining and, in the insets, TUNEL and Gomori methenamine-silver stainings), g histological score (n = 6 mice/group) and h number of TUNEL-positive cells (n = 5 mice/group). i T helper-related gene expression in thoracic lymph nodes (RT- PCR) and cytokine production (ELISA) (n = 3–7 mice/group). Hoechst was used for nuclear counterstain in blue. Photographs were taken with a high-resolution microscope (Olympus BX51), ×4 and ×100 magnification (scale bars 1000 µm and 20 µm, respectively). Data are presented as mean ± SEM and representative of three experiments. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001, Tph1−/− vs Tph1+/+ mice. a, b, d Two-way ANOVA, Sidak post test. Adjusted P values for a: 0.0003; b, left panel: <0.0001; b, right panel: <0.0001 (3 dpi), 0.0313 (7 dpi); d: 0.0003. e Mann–Whitney test, two-tailed. P value for phagocytosis: 0.0022 (Macrophages), 0.0043 (Neutrophils); Unpaired t test, two-tailed. P value for DHE: 0.0032 (Macrophages), g Kruskal–Wallis test. P value: <0.0001. h Two-way ANOVA, Sidak post test. Adjusted P values: 0.0291 (3 dpi), 0.0001 (7 dpi); i Two-way ANOVA, Sidak post-test. Adjusted P values: 0.024 (Tbet), 0.0343 (IFN-γ, 3 dpi), 0.0037 (IFN-γ, 7 dpi), 0.0001 (Gata3), 0.0458 (IL-4), 0.0212 (IL-17A), 0.0027 (Foxp3), 0.0366 (IL-10, 3 dpi), 0.0005 (IL-10, 7 dpi), 0.0023 (IL-9). Source Data are provided as a Source Data file.
