Fig. 3: Mast cells produce 5-HT in A. fumigatus infection.
From: Bridging of host-microbiota tryptophan partitioning by the serotonin pathway in fungal pneumonia
a The tryptophan hydroxylase (TPH)1 pathway (DDC, dopa decarboxylase; AANAT, arylalkylamine N-acetyltransferase; ASMT, N-acetylserotonin O-methyltransferase; MAO, monoamine oxidase; ALDH, aldehyde dehydrogenase). Tph1+/+ and Tph1–/– littermates were infected intranasally with A. fumigatus and assessed at 3 or 7 days post infection (dpi) for b lung gene expression (RT-PCR) (n = 4–6 mice/group), c levels of 5-HTP and 5-HT in perfused lungs and serum (n = 3–6 mice/group) and d immunofluorescence staining of 5-HT, pulmonary neuroepithelial bodies (Pgp9.5) and mast cells (chymase) in perfused lungs. White arrows indicate 5-HT+ cells. Hoechst was used for nuclear counterstain in blue. Photographs were taken with a high-resolution microscope (Olympus BX51), ×100 magnification (scale bars 20 µm). Data are expressed as mean ± SEM and representative from three experiments. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. Two-way ANOVA, Tukey post hoc test. Adjusted P values for b: for Tph1, 0.0273 (Tph1+/+, 0 vs 3 dpi), 0.0010 (Tph1+/+, 3 vs 7 dpi), 0.0007 (3 dpi, Tph1+/+ vs Tph1–/–); for Ddc, 0.0013 (Tph1+/+, 0 vs 3 dpi), 0.0084 (Tph1+/+, 3 vs 7 dpi), 0.0004 (3 dpi, Tph1+/+ vs Tph1–/–); for Aanat, 0.0121 (Tph1+/+, 0 vs 7 dpi), 0.0027 (7 dpi, Tph1+/+ vs Tph1–/–); for Maoa, 0.0217 (3 dpi, Tph1+/+ vs Tph1–/–); for Sert, 0.0131 (3 dpi, Tph1+/+ vs Tph1–/–), 0.0343 (7 dpi, Tph1+/+ vs Tph1–/–); for c: for 5-HTP, 0.0019 (3 dpi, Tph1+/+ vs Tph1–/–), 0.0055 (Tph1+/+, 3 vs 7 dpi), 0.038 (Tph1+/+, 0 vs 3 dpi); for lung 5-HT, 0.0069 (0 dpi, Tph1+/+ vs Tph1–/–), < 0.0001 (3 dpi, Tph1+/+ vs Tph1–/–), 0.0086 (7 dpi, Tph1+/+ vs Tph1–/–), 0.0071 (Tph1+/+, 3 vs 7 dpi), 0.0456 (Tph1+/+, 0 vs 3 dpi). Source Data are provided as a Source Data file.
