Fig. 4: 5-HT coordinates the IDO1/AhR axis in lung pneumonia.
From: Bridging of host-microbiota tryptophan partitioning by the serotonin pathway in fungal pneumonia
Tph1+/+ and Tph1–/– littermates were infected intranasally with A. fumigatus and lungs were assessed at 3 or 7 days post infection (dpi) for a IDO1 expression by immunofluorescence staining and b western blotting, c Haao and Kmo expression, d Tdo2 expression (also in the liver), e AhR expression by immunofluorescence staining and f western blotting, g Cyp1a1 and Cyp1b1 expression and h AhR activity (luciferase assay on H1L6.1 cells exposed to lung homogenates from infected mice). c, d, g, h (n = 4 mice/group in 0 dpi and n = 6 mice/group in 3 and 7 dpi), i Lung gene expression in mice treated with parachlorophenylalanine (PCPA) or 5-Hydroxytryptophan (5-HTP) as in legend to Fig. 2 (n = 4 mice/group in None and n = 5 mice/group in treated mice). Hoechst was used for nuclear counterstain in blue. Photographs were taken with a high-resolution microscope (Olympus BX51), ×40 and ×100 magnification (scale bars 100 and 20 µm, respectively). Data are expressed as mean ± SEM and representative from three experiments. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001, Tph1–/– vs Tph1+/+ mice, infected vs uninfected (none) mice, treated vs untreated mice. One-way or two-way ANOVA, Turkey post hoc test. Adjusted P values for c: for Haao, 0.0196 (Tph1+/+, 0 vs 7 dpi), 0.0249 (7 dpi, Tph1+/+ vs Tph1–/–); for Kmo, 0.0003 (Tph1+/+, 0 vs 7 dpi), <0.0001 (7 dpi, Tph1+/+ vs Tph1–/–); for g: for Cyp1a1, 0.0055 (Tph1–/–, 0 vs 7 dpi), 0.0003 (7 dpi, Tph1+/+ vs Tph1–/–); for Cyp1b1, 0.0384 (Tph1–/–, 0 vs 7 dpi), 0.0265 (7 dpi, Tph1+/+ vs Tph1–/–); for h: 0.0381 (Tph1–/–, 0 vs 7 dpi), 0.0021 (7 dpi, Tph1+/+ vs Tph1–/–). i Unpaired t test, two-tailed, P values in treated vs None mice: for Ido1, 0.0165 (Tph1+/+), 0.0114 (Tph1–/–); for Haao, 0.0019 (Tph1–/–); for Cyp1a1, <0.0001 (Tph1+/+), for Cyp1b1, 0.0103 (Tph1–/–). Source Data are provided as a Source Data file.
