Fig. 6: MB299B neurons display Ca2+rhythms.
From: Circadian clock disruption promotes the degeneration of dopaminergic neurons in male Drosophila
a Projections of the LNds contact dendritic arbors of MB299B neurons. Image created using the hemibrain connectome data46 via the NeuPrint tool47. b Illustration of the method for live GCaMP imaging. c, d MB299B > GCaMP7s fluorescence levels in MB299B neurons throughout 24 h on the first day in DD following LD-entrainment in w1118 (c) and per0 (d). Relative fluorescence intensity (mean ± SEM) was determined from three independent experiments. In (c), the analysis included an average of 79 (53–146 cells) cells per timepoint from 5–10 w1118 flies (see Source Data for individual sample numbers per timepoint). In (d), an average of 76 (44–131) cells per timepoint from 5–10 per0 flies were analyzed (see Source Data for individual sample numbers per timepoint). Source data are provided as a Source Data file.
