Fig. 1: The 15–30 nt sRNAs from mouse liver mainly end with 3′-cP.
From: Discovery of the major 15–30 nt mammalian small RNAs, their biogenesis and function
a, b Only a small fraction of the 15–30 nt sRNAs from mouse liver can be ligated to the adapter App-DNA3-ddC by RNA ligase T4 Rnl2 (a, n = 3) or polyadenylated by Poly(A) polymerase (PAP) (b, n = 4). c LC–MS/MS analysis of modified nucleosides and nucleotides from an enzymatic digestion of the 15–30 nt sRNAs. Numbered peaks are 1, m1A; 2, 2′,3′-cUMP; 3, 2′,3′-cGMP; 4, 2′,3′-cAMP; 5, 2′,3′-cCMP; 6, m6A; 7, m3C; 8, ψ; 9, I; 10, m5C; 11, Um; 12, m22G n = 3. d The abundance of the 15–30 nt sRNAs with 3′-cP when analyzed by LC–MS/MS n = 3. e, f The 3′-end of the 15–30 nt sRNAs is mainly cP when analyzed by ligation assay (e) or polyadenylation assay (f) n = 3. g The abundance of the 15–30 nt sRNAs with 3′-OH and 3′-cP when analyzed in (e, f) n = 3. Data are presented as mean ± SD, statistical significance was determined by two-tailed Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001. ns not significant. Exact P values can be found in Source Data Fig. 1. Source data are provided as a Source data file.
