Fig. 2: TANT-seq reveals many abundant 15–30 nt mammalian sRNA biotype classes with 3′-cP and 3′-OH.
From: Discovery of the major 15–30 nt mammalian small RNAs, their biogenesis and function
a Schematic of the workflow for constructing a TANT-seq library to simultaneously profile sRNAs with 3′-OH and 3′-cP. b The relative abundance of 15–30 nt sRNAs with 3′-OH or 3′-cP from mouse liver when detected by TANT-seq n = 3. c TANT-seq reveals many abundant sRNA biotype classes with 3′-OH or 3′-cP in mouse liver n = 3. d Proportion of sRNA categories detected by TANT-seq. #, indicates a dramatic change between 3′-OH and 3′-cP sublibraries. e Length distribution and abundance of 15–30 nt sRNAs in mouse liver. f The percentage of tsRNA classified into each subgroups in 3′-OH and 3′-cP sublibraries is significantly different n = 3. g The total or top 1000 unique sRNAs between 3′-OH and 3′-cP sublibraries only have a little overlap. Data are presented as mean ± SD, statistical significance was determined by two-tailed Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001. Exact P values can be found in Source Data Fig. 2. Source data are provided as a Source data file.
