Fig. 3: Validation of the TANT-seq data by TE-qPCR and Northern blot.
From: Discovery of the major 15–30 nt mammalian small RNAs, their biogenesis and function
a Schematic representation of TE-qPCR to detect sRNAs with 3′-OH, 3′-P or 3′-cP. b The indicated 9 synthetic sRNAs were mixed and detected by TE-qPCR n = 3. c, d The indicated sRNAs in mouse liver (c), Hepa 1–6 and NIH/3T3 cells (d) were detected by TE-qPCR n = 3. e The indicated sRNAs in mouse liver were detected by Northern blot after treatment with or without AP or T4 Pnk and ligation with or without T4 Rnl2, and the remaining sRNAs were quantified n = 3. f The indicated sRNAs in liver of wild-type mice fed, fasted or fed with high-fat diet, or in liver of db/db mice were detected by TE-qPCR n = 6. Data are presented as mean ± SD. Statistical significance was determined by two-tailed Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001. Exact P values can be found in Source Data Fig. 3. Source data are provided as a Source data file.
