Fig. 4: The biogenesis of sRNA-cPs partially depends on Ang and RNase 4.

a Nucleotide enrichment graph for 15–30 nt sRNA-cPs from mouse liver. b The relative RNA level detected by TE-qPCR in mouse liver total RNA after digestion with RNase A or RNase T1. Veh, vehicle n = 3. c The expression level of RNase A family members in mouse liver n = 3. d The relative RNA level detected by TE-qPCR in mouse liver total RNA after digestion with RNase A, Ang or RNase 4 n = 3. e Northern blot analysis of the sRNA level in mouse liver total RNA after digestion with Ang or RNase 4. Arrowhead indicates the band with expected size. f, g Transfection with Ang or RNase 4 markedly increased the level of indicated sRNA-cPs in RNH1 KO Hepa 1–6 cells when detected by TE-qPCR (f) or Northern blot (g) n = 3. h, i The level of indicated sRNAs in WT, Ang KO (AKO), RNase 4 KO (RKO) and Ang/RNase 4 double KO (DKO) Hepa 1–6 cells pretreated with RNase inhibitor when detected by TE-qPCR (h) or Northern blot (i) n = 3. j Volcano plots showing differentially expressed 15–30 nt sRNAs with 3′-OH or 3′-cP from WT, AKO, RKO or DKO Hepa 1–6 cells when detected by TANT-seq. k The level of indicated sRNAs in WT, AKO, RKO and DKO Hep G2 cells pretreated with RNase inhibitor when detected by TE-qPCR n = 3. Data are presented as mean ± SD. Statistical significance was determined by two-tailed Student’s t-test, except in (j) adjusted P value was calculated by Benjamini-Yekutieli method. a P < 0.05; b P < 0.01; c P < 0.001. Exact P values can be found in Source Data Fig. 4. Source data are provided as a Source data file.