Fig. 3: Dorsal striatum expression of the dLight sensor on plasma membrane of dendrites synapsing with TH-axons. | Nature Communications

Fig. 3: Dorsal striatum expression of the dLight sensor on plasma membrane of dendrites synapsing with TH-axons.

From: Distinct sub-second dopamine signaling in dorsolateral striatum measured by a genetically-encoded fluorescent sensor

Fig. 3: Dorsal striatum expression of the dLight sensor on plasma membrane of dendrites synapsing with TH-axons.

a Immunofluorescence detection of dLight sensor with an antibody targeted to the GFP moiety contained in the sensor (cyan) and tyrosine hydroxylase (TH, red) in the dorsal striatum from a mouse injected with a viral vector encoding dLight sensor. Apposition of a dLight-positive dendrite and TH-positive axon is seen at high magnification (right panels). b Electron micrographs of immunogold detection of dLight sensor (gold particles, cyan arrowheads) on the plasma membrane of a dendrite (cyan outline in b). Note the apposition of dendritic dLight and a presynaptic axon (red arrow in b). c dLight expression on the plasma membrane of a soma (cyan outline). Note also the dLight sensor (gold particles) in association with Golgi apparatus and endoplasmic reticulum (ER). d Detection of dLight sensor (scattered dark material, cyan arrowheads) in a dendrite (cyan outline) establishing synapses (red arrows) with a TH-positive axon (gold particles, red arrowheads). Quantitative GFP data (n = 3 cases) is presented in Supplementary Table S1.

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