Fig. 4: E2F7 and RCOR mediate the effect of RB1 loss on YAP downregulation.
From: YAP silencing by RB1 mutation is essential for small-cell lung cancer metastasis
a Mass spectrometry showing the Flag-E2F7 interacting proteins in H526 cells. The background of proteins recovered from the Flag vector control expressing cells has been subtracted. The 10 top enriched proteins are listed. b E2F7 interacts with RCOR1. Co-immunoprecipitation (Co-IP) experiment confirmed the interaction between Flag-tagged E2F7 and endogenous RCOR1 in H209 and H69 cells. c RCOR1 interacts with E2F7 but not E2F1. Co-IP showed the interaction between Flag-RCOR1 and endogenous E2F family proteins in H209 and H69 cells. d RCOR1/2/3 KO induces YAP mRNA and protein in H209 and H69 cells. e RCOR1/2/3 and E2F7/8 KO increases H3K4me3 YAP promoter of H209 and H69 cells. ChIP and RT-qPCR show YAP promoter H3K4 trimethylation. f YAP is required for RB1 to inhibit ameboid migration. Cell morphology (left) and migration (right) of control, RB1 rescued, YAP KO, and YAP KO and RB1 rescued H209 cells under confinement. g E2F7/8 KO or RCOR1/2/3 KO inhibits ameboid migration. Control, E2F7/8 KO, and RCOR1/2/3 KO H209 cells under confinement. h REST expression of SCLC cell lines and others in CCLE database. i Ectopic expression of REST in H209 and H69 cells induces YAP expression and abolishes the effect of RB. Source data are provided as a Source data file.
