Fig. 5: Antibody responses to different MPXV antigens longitudinally in IMVANEX-vaccinated individuals.

Longitudinal sampling of individuals receiving IMAVENEX vaccination against: a a panel of 24 MPXV and 3 VACV antigens or the pooled antigen ELISA. Bars represent the mean ± standard error of the mean. n is equal to the number of biologically independent samples. b Evaluation of the pooled antigen ELISA to determine sensitivity and specificity. Bars represent the mean ± standard deviation. Each dot represents a biologically independent sample. c Using the pooled antigen ELISA, endpoint titres were determined for positive groups. Bars represent the mean ± standard error of the mean. Each dot represents a biologically independent sample. Comparison of the ELISA results of MPXV-infected and Smallpox-vaccinated serum samples using individual antigens relative to the pool of antigens (d) split by antigen, or e linear regression of individual antigen results relative to pooled antigen ELISA results (n = 121 biologically independent samples). Dotted lines represent the 95% confidence intervals. Black lines represent X = Y. P values for d were generated using a paired t test and each pair of dots joined by a line represents a biologically independent sample.