Table 3 Statistical analysis of individual MPXV/VACV antigens in detecting Smallpox- or MPXV-specific antibody responses

From: Monkeypox virus-infected individuals mount comparable humoral immune responses as Smallpox-vaccinated individuals

Virus	Protein	Area under the ROC curve (both)										ROC analysis (versus negative)
		Area under the ROC curve (both)										Smallpox vaccine					Mpox convalescent					Both
		Total area	Std. error	95% confidence interval	P value	PD1 D24	PD2 D14	PD2 D43	PD2 D63	MPX	Cutoff		Sensitivity (%)	Specificity (%)	LR	Cutoff		Sensitivity (%)	Specificity (%)	LR	Cutoff		Sensitivity (%)	Specificity (%)	LR
		Total area	Std. error	95% confidence interval	P value	PD1 D24	PD2 D14	PD2 D43	PD2 D63	≥2022	Cutoff		Sensitivity (%)	Specificity (%)	LR	Cutoff		Sensitivity (%)	Specificity (%)	LR	Cutoff		Sensitivity (%)	Specificity (%)	LR
MPXV	A5	0.7064	0.04359	0.6210 to 0.7919	<0.0001	0.998	0.2562	0.9744	0.9985	<0.0001	>0.3038		8.333	98.41	5.25	>0.1903		34.78	95.24	7.304	>0.1903		24.39	95.24	5.122
	A14	0.6639	0.04553	0.5746 to 0.7531	0.0007	0.995	0.9392	0.4461	0.9814	0.0041	>0.2849		8.333	98.41	5.25	>0.1828		23.91	95.24	5.022	>0.2661		9.756	98.41	6.146
	A26	0.5494	0.04802	0.4552 to 0.6435	0.3091	0.9283	0.9987	0.9977	0.9918	0.0413	<0.1448		100	4.762	1.05	>0.1233		17.39	95.24	3.652	>0.1233		10.98	95.24	2.305
	A27	0.9909	0.005213	0.9807 to 1.000	<0.0001	0.0394	0.9989	0.994	0.9981	<0.0001	>0.1701		11.11	98.48	7.306	>0.1838		91.3	98.86	80.04	>0.1383		58.54	96.58	17.11
	A29	0.7243	0.0414	0.6431 to 0.8054	<0.0001	0.9998	0.0072	0.9999	>0.9999	<0.0001	>0.2443		8.333	98.41	5.25	>0.1344		71.74	93.65	11.3	>0.1475		42.68	95.24	8.963
	A33	0.6155	0.04695	0.5235 to 0.7075	0.0173	0.9743	0.677	0.4456	0.0833	0.0037	>0.1127		8.333	96.83	2.625	>0.1135		23.91	96.83	7.533	>0.1266		12.2	98.41	7.683
	A35	0.8878	0.02824	0.8325 to 0.9432	<0.0001	0.9996	<0.0001	0.1738	0.9561	<0.0001	>0.2443		61.11	93.65	9.625	>0.2423		93.48	93.65	14.72	>0.2423		79.27	93.65	12.48
	A36	0.5505	0.04877	0.4549 to 0.6461	0.2978	>0.9999	0.5128	0.7373	0.9058	0.5869	>0.1365		50	76.19	2.1	<0.3010		97.83	15.87	1.163	>0.1055		46.34	71.43	1.622
	A44	0.567	0.04769	0.4735 to 0.6605	0.1676	0.6103	0.6583	>0.9999	0.9997	0.13	<0.1384		94.44	9.524	1.044	>0.1254		28.26	90.48	2.967	>0.1232		19.51	90.48	2.049
	B2	0.9743	0.0121	0.9505 to 0.9980	<0.0001	0.4434	<0.0001	<0.0001	0.0029	<0.0001	>0.1827		91.67	87.3	7.219	>0.2980		93.48	93.65	14.72	>0.3437		89.02	95.24	18.7
	B5	0.651	0.04596	0.5609 to 0.7411	0.0019	0.9944	0.0023	0.9386	0.9969	0.7119	>0.1165		75	58.73	1.817	>0.1610		56.52	69.84	1.874	>0.1275		64.63	61.9	1.697
	B6	0.9383	0.02286	0.8934 to 0.9831	<0.0001	0.9997	<0.0001	<0.0001	0.0078	<0.0001	>0.1830		77.78	95.24	16.33	>0.1932		89.13	95.24	18.72	>0.1830		84.15	95.24	17.67
	C15	0.5407	0.04873	0.4452 to 0.6362	0.4045	0.9955	0.9997	0.9222	0.2299	0.9961	<0.1354		97.22	11.11	1.094	<0.1382		95.45	11.11	1.074	<0.1382		96.25	11.11	1.083
	C18*	0.6045	0.04828	0.5098 to 0.6991	0.0323	0.9999	0.9999	>0.9999	0.9612	0.2184	>0.3874		88.89	31.75	1.302	>0.3943		88.64	31.75	1.299	>0.3874		88.75	31.75	1.3
	C19	0.5853	0.04734	0.4925 to 0.6781	0.0789	0.8182	0.9722	0.8888	0.0017	0.3028	>0.1193		2.778	98.41	1.75	>0.1193		6.522	98.41	4.109	>0.1193		4.878	98.41	3.073
	D13	0.6453	0.04581	0.5555 to 0.7351	0.0028	0.9998	0.9982	0.5232	0.9976	0.0006	>0.1205		13.89	92.06	1.75	>0.1163		34.78	92.06	4.383	>0.1163		25.61	92.06	3.227
	D14	0.5681	0.04849	0.4731 to 0.6632	0.1603	0.9997	0.9999	0.9999	0.9997	0.4863	>0.1295		0	98.41	0	>0.1035		8.696	96.83	2.739	>0.1035		4.878	96.83	1.537
	E8	0.9123	0.02532	0.8627 to 0.9619	<0.0001	0.9999	<0.0001	<0.0001	0.0118	<0.0001	>0.1406		72.22	98.41	45.5	>0.1357		80.43	98.41	50.67	>0.1357		76.83	98.41	48.4
	F3	0.6087	0.0476	0.5154 to 0.7020	0.0259	>0.9999	0.6754	0.9713	0.968	0.0003	>0.1067		5.556	98.41	3.5	>0.1071		22.73	98.41	14.32	>0.1067		15	98.41	9.45
	H3	0.6998	0.04355	0.6144 to 0.7851	<0.0001	0.9983	0.3433	0.1102	0.8603	0.0185	>0.1125		30.56	88.89	2.75	>0.1349		60.87	92.06	7.67	>0.1125		48.78	88.89	4.39
	I1	0.6066	0.0514	0.5059 to 0.7074	0.0395	0.4004	0.302	0.4065	0.4221	0.9858	<0.1253		100	1.961	1.02	<0.1099		100	3.922	1.041	<0.1099		100	3.922	1.041
	L1	0.5136	0.04843	0.4187 to 0.6086	0.7786	0.9364	>0.9999	0.1092	0.9347	0.9707	<0.1615		97.22	1.587	0.9879	>0.1052		6.522	95.24	1.37	>0.1388		6.098	98.41	3.841
	L4	0.5124	0.04802	0.4183 to 0.6065	0.7985	0.0015	0.6977	0.9759	0.9874	0.5095	>0.1263		13.89	98.41	8.75	<0.2919		97.83	0	0.9783	>0.1053		15.85	96.83	4.994
	M1	0.8097	0.05218	0.7074 to 0.9120	<0.0001	0.9997	<0.0001	<0.0001	<0.0001	0.1207	>0.1164		63.89	98.01	32.07	>0.1154		11.11	98.01	5.578	>0.1154		34.57	98.01	17.35
VACV	A27	0.7754	0.03866	0.6996 to 0.8511	<0.0001	0.9998	0.4736	0.6861	0.9869	<0.0001	>0.1352		38.89	72.58	1.418	>0.3706		80.43	90.32	8.312	>0.3706		53.66	90.32	5.545
	A33	0.9185	0.0252	0.8691 to 0.9679	<0.0001	0.9998	<0.0001	<0.0001	0.0449	<0.0001	>0.2106		75	94	12.5	>0.1387		84.78	94	14.13	>0.1387		80.49	94	13.41
	B5	0.9408	0.02086	0.8999 to 0.9817	<0.0001	0.983	<0.0001	<0.0001	<0.0001	<0.0001	>0.1657		86.11	92.06	10.85	>0.1138		86.96	90.48	9.13	>0.1138		87.8	90.48	9.22
Pooled antigen ELISA		0.9834	0.01037	0.9630 to 1.000	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001	>0.1926		97.22	98.22	54.77	>0.2350		95.65	98.22	53.88	>0.1926		96.34	98.22	54.27

ROC analysis was performed for each assay (single antigen and pooled antigens) comparing the absorbance values of all negative samples versus either post-Smallpox vaccine, mpox convalescent or both sample groups combined. The sensitivity, specificity and likelihood ratio (LR) values for each group, alone or combined, are reported for chosen absorbance cutoffs. Area under the ROC curve values are reported for both sample groups combined apart from MPXV A27 and MPXV M1R where values for mpox convalescent samples only and post-Smallpox vaccine samples only are reported, respectively. Dunnett’s multiple comparisons test was used for each group, relative to the negative control samples.* - non-specific binding was observed to C18.

Back to article page

Table 3 Statistical analysis of individual MPXV/VACV antigens in detecting Smallpox- or MPXV-specific antibody responses

Search

Quick links