Fig. 1: Knock-in (KI) mice in which Serine (Ser) 14 of Bcl-xL is replaced with Alanine show worse phenotypes in response to pressure overload.

a Representative immunoblots showing phosphorylation of Bcl-xL at Ser14 in the heart with time course after transverse aortic constriction (TAC). Sham is a heart sample collected one hour after sham surgery. h; hours, and d; days after TAC. Lower panel shows densitometric analysis of relative expression of pBcl-xL (Ser14)/Bcl-xL in the heart. Kruskal-Wallis test with sham as the control. p values are shown in the figure (n = 5). b Kaplan–Meier survival curves. Log-rank (Mantel-Cox) test p = 0.0006. n = 25–36. Lower panel shows the survival curves 1 week after TAC. c Representative pictures of M-mode echocardiography. Yellow lines indicate left ventricular (LV) end-systolic and -diastolic diameters. Vertical scale bar indicates 5 mm and horizontal scale bar indicates 100 ms. d Ejection fraction (EF) with time course after TAC or sham surgery. Two-way ANOVA with Tukey’s multiple comparison test. ****p < 0.0001 compared to +/+ (wild type) control. #p = 0.0183 and ####p < 0.0001 compared to knock-in/+. &&&&p < 0.0001, &&&p = 0.0003, && p = 0.0025, and &p = 0.0141 compared to Sham (upaired t test or Mann–Whitney test, two-sided). n = 6–16 (TAC) and 5 (Sham). e LV end-diastolic pressure at the indicated time points after TAC or sham surgery, evaluated by hemodynamic study. Two-way ANOVA with Tukey’s multiple comparison test. **** p < 0.0001. n = 5. f Lung weight normalized by tibia length at the indicated time points after TAC or sham surgery. Two-way ANOVA with Tukey’s multiple comparison test. ****p < 0.0001, ***p < 0.001, and **p < 0.01. Adjusted p values for (f): 0.0004 (1 W, +/+ vs KI/ + ), 0.0018 (2 W, +/+ vs KI/ + ), 0.0008 (2 W, +/+ vs KI/KI). n = 5–8. N represents biologically independent replicates. Data are mean ± SEM. Source data are provided as a Source Data file.