Fig. 2: ECM landscape of normal and fibrotic lung revealed by high-dimensional imaging mass cytometry.
From: Lung extracellular matrix modulates KRT5+ basal cell activity in pulmonary fibrosis
a Imaging mass cytometry (IMC) of distal lung tissue from normal control (n = 1, H&E overview shown). Left panel; distribution of ECM components; collagen I (blue), collagen III (magenta) and versican (green). Right panel; fibronectin (yellow) and collagen IV (turquoise) in relation to KRT5+ BCs (red) and nuclear DNA marker (white). Representative image demonstrating key regions; airway (AW), peribronchial (PB) and alveolar (ALV). b–d IMC of distal lung tissue from a patient with IPF (n = 1, H&E overview shown) showing distinct regions; b peribronchial and perivascular space (PVS), c alveolar and fibrotic interstitium (FIBRO), and d honeycomb cyst (HC). Upper panel; collagen I (blue), collagen III (magenta), versican (green) and KRT5+ BCs (red). Lower panel; fibronectin (yellow), collagen IV (turquoise), KRT5+ BCs (red) and nuclear DNA marker (white). Scale bar, 150 μm.
