Fig. 7: SPARC restricts KRT5+ BC migration.
From: Lung extracellular matrix modulates KRT5+ basal cell activity in pulmonary fibrosis
a Individual cell track trajectories for healthy KRT5+ BCs on collagen I (blue) or collagen I and SPARC (green), Scale bar, 100 μm b Cell tracks plotted from centroid; Scale bar, 100 μm. c Mean square displacement (MSD) over time of all healthy KRT5+ BCs on collagen I (blue) or collagen I and SPARC (green) (mean ± SEM). d–f Beeswarm SuperPlots showing migration data for KRT5+ BCs from healthy controls (n = 6, in triplicate) cultured in wells with no coating (magenta), collagen I (blue) or collagen I plus SPARC 20 µg/ml (green); d displacement over 12 h (BCs on no coating vs. collagen I, P value = 0.0021; BCs on collagen I vs. collagen I plus SPARC, P value = 0.0205), e mean track speed (BCs on no coating vs. collagen I plus SPARC, P value = 0.0166), and f straightness ratio (BCs on no coating vs. collagen I, P value < 0.0001; BCs on no coating vs. collagen I plus SPARC, P value = 0.0132; BCs on collagen I vs. collagen I plus SPARC, P value = 0.0108). Each small dot represents cell-level data which is colour coded according to biological replicate. The larger circles represent the mean value per biological replicate. Summary statistics with mean and standard deviation are superimposed on the plot. Sample-level means compared using a one-way ANOVA with Tukey’s multiple comparison test, ****P < 0.0001, **P < 0.01, *P < 0.05. Source data are provided as a Source Data file.
