Fig. 6: The C19F variant in MGP causes ER stress and cell death in HEK-293 cells. | Nature Communications

Fig. 6: The C19F variant in MGP causes ER stress and cell death in HEK-293 cells.

From: Specific heterozygous variants in MGP lead to endoplasmic reticulum stress and cause spondyloepiphyseal dysplasia

Fig. 6

a Immunofluorescence imaging using anti-FLAG and anti-calnexin antibodies shows the upregulation of calnexin in HEK-293 cells expressing C19F MGP. DAPI stains the nuclei (blue) (n = 4 individual image fields/group). b Immunofluorescence imaging using an anti-CHOP antibody shows the upregulation of CHOP in cells expressing C19F MGP. DAPI stains the nuclei (n = 4 individual image fields/group). c Confocal immunofluorescence images of transfected HEK-293 cells stained with anti-FLAG and anti-calnexin antibodies show that FLAG-tagged C19F MGP protein is co-localized in the ER with the stress marker calnexin. d Cell death assay. Ethidium homodimer-stained (red) transfected HEK-293 cells show a significant increase in the number of dead cells expressing C19F MGP when compared to those expressing WT MGP (n = 10 individual image fields/group). e TUNEL assay shows increased apoptosis in C19F MGP expressing cells (n = 5 individual image fields/group). Data presented as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 vs. WT by two tailed unpaired t test. Actual P values and source data are provided as a Source Data file.

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