Fig. 2: Neuronal maturation and migration are relatively more advanced in dunnarts than mice.

a, b Cortical images (a) and quantification of leading edge of migrating cells distribution (b) in the primary somatosensory cortex of mice and dunnarts electroporated at S20 (labeling DL neurons) and collected between S21 and S26. N for MS S21:5, S22:5, S23:9, S24:3, S26:3; FTD S21:20, S22:13, S23:3, S24:3, S26:3. c, d Cortical images (c) and quantification of the leading edge of migrating cells distribution (d) in the primary somatosensory cortex of mice and dunnarts electroporated at S23 (labeling UL neurons) and collected between S24 and S28. N for MS S24:9, S25:8, S26:5, S27:4, S28:4; FTD S24:7, S25:9, S26:4, S27:5, S28:7. e, f Quantification of the proportion of electroporated TdTom⁺ cells in each compartment of the cortex following electroporation at S20 (e) or S23 (f) and subsequent collection across a range of developmental stages. N for MS in (e) S21:5, S22:5, S23:9, S24:3, S26:3; FTD in (e) S21:21, S22:13, S23:3, S24:3, S26:3. N for MS in (f) S24:9, S25:8, S26:5, S27:4, S28:4; FTD in (f) S24:7, S25:9, S26:4, S27:5, S28:7. g, h Images of mouse (g) and dunnart (h) cortex electroporated at S21 and collected at S22 colabeled with NEUROD1 (ND1, red) and SOX2 (blue). i Quantification of colabeled TdTom⁺NEUROD1⁺ cells as a proportion of the colabeled TdTom⁺SOX2⁺ cells in the cortex of each species electroporated at S21 and collected at S22. N for MS and FTD:4. Data are presented as mean values ± SEM and were compared using Mann-Whitney U tests (b, d), MANOVA or Npmv followed by pairwise log ratio comparisons (e, f) or t-test (i); *p < 0.05, **p < 0.01, ***p < 0.001. See Supplementary Table 3 for exact p values and statistical test details. CP cortical plate, DL deep layers, FTD fat-tailed dunnart, IZ intermediate zone, L layer, MS mouse, MZ marginal zone, S stage, SVZ subventricular zone, UL upper layers, VZ ventricular zone. Scale bars: a, b S21-25 = 100 µm; S26-28 = 200 µm; g, h = 25 µm.