Fig. 6: Analysis of endometrial epithelial organoids derived from the window of implantation controls and Asherman´s syndrome patients.

A Scheme of EEO generation and analysis. B Representative images of EEOs generated from AS endometria (AS EEO, n = 3) at different passages (P0, 1, 2 = Passage 0, 1, 2) and WOI controls (Control EEO, n = 3). C UMAP integration of main cell types detected in EEOs derived from AS patient and WOI control endometria (AS EEOs, 14,459 cells; control EEOs, 15,594 cells). D Cell type distribution across groups in EEOs generated from AS patient and WOI control endometria. E Dot plot displaying the expression of selected genes that distinguish the main EEO cell populations. F Polygonal representation of cell-type identity probabilities predicted by a logistic model trained on epithelial cell subtypes from in vivo WOI samples. G Box plot prediction scores for each cell type and organoid group. H Differential gene expression dot plots for epithelial glandular subtypes in AS EEOs and control EEOs (two-sided Wilcoxon Rank Sum test; FDR < 0.05). Dunn test applied to evaluate differences between groups; two-side Dunn test was applied to evaluate differences between groups; p-values were adjusted using the Bonferroni method (n = 3 independent samples for each group); **p < 0.01; ****p < 0.0001. AS Asherman’s syndrome, EEO endometrial epithelial organoids, Epi epithelial, P passage.