Fig. 3: PYCR1 facilitates ELK4-mediated transcriptional repression for growth maintenance.

a–f HCT116 cells expressing the indicated Flag-rPYCR1 were collected. b, c, e, f cells were simultaneously transfected with or without ELK4 siRNA. b, c mRNA levels of indicated ELK4 target genes were analyzed by real-time PCR. d–f ChIP analysis were performed using the indicated antibody; the primers covering the ELK4-binding sites at promoter regions of the indicated genes were utilized for real-time PCR analysis. The y axis shows the value normalized to the input. a differential expression analysis was performed using the edgeR. b–f data are presented as the mean ± s.d. (n = 4 independent experiments); statistical analysis was performed using the two-tailed Student’s t test. Source data are provided as Source Data Fig. 3. a The cDNA microarray analysis was performed for cells cultured under hypoxia for 24 h. Among genes that were expressed differentially between two groups, the genes whose promoter contained the ELK4-binding consensus were presented. b, c Cells expressing WT rPYCR1 and rPYCR1 Y135F (b) or T238A (c) were cultured under hypoxia for 24 h. d Cells expressing the indicated Flag-rPYCR1 were cultured under hypoxia for 12 h. e, f Cells expressing WT rPYCR1 and rPYCR1 Y135F (e) or T238A (f) were cultured under hypoxia for 12 h.