Fig. 4: PYCR1 facilitates Sirt7-mediated H3K18 deacetylation.

a, e immunoprecipitation and immunoblotting analysis were performed with indicated antibodies. d, h, i ChIP analysis were performed with indicated antibodies, the primers covering the ELK4-binding sites at promoter regions of the indicated genes were utilized for real-time PCR analysis. The y axis shows the value normalized to the input. b, c, f, g mRNA levels of indicated ELK4 target genes were analyzed by real-time PCR. b–d, f–i data are presented as the mean ± s.d. (n = 4 independent experiments); statistical analysis was performed using the two-tailed Student’s t test. Source data are provided as Source Data uncropped western blots and Source Data Fig. 4. a HCT116 cells expressing indicated Flag-PYCR1s were cultured under normoxia or hypoxia for 12 h. b, c HCT116 cells expressing WT rPYCR1 and rPYCR1 Y135F (b) or T238A (c) were transfected with or without Sirt7 siRNA. Cells were cultured under normoxia or hypoxia for 24 h. d HCT116 cells expressing the indicated Flag-rPYCR1s were transfected with or without ELK4 siRNA. Cells were cultured under normoxia or hypoxia for 12 h. e HCT116 cells expressing the indicated Flag-rPYCR1s were overexpressed with WT Histone H3 or Histone H3K18R. f, g HCT116 cells transfected with or without Sirt7 siRNA (f) or expressing indicated Flag-rPYCR1s (g) were overexpressed with WT Histone H3 or Histone H3K18R. Cells were cultured under normoxia or hypoxia for 24 h. h, i HCT116 cells transfected with or without Sirt7 siRNA (h) or expressing indicated Flag-rPYCR1s (i) were cultured under normoxia or hypoxia for 12 h.