Fig. 2: Inflammation-related pathways are enriched at the intestinal stem cell level.

a Hierarchical clustering and heatmap of the Pearson correlation of the RNA-seq datasets of Lgr5^hi intestinal stem cell for indicated ages. n = 3 mice per group were analyzed. b Venn diagram of the DEGs found in the RNA-seq of the Lgr5^hi intestinal stem cells for the indicated genders. P value was calculated by two-sided hypergeometric distribution test. c Scatter plot of the −log10 p values of the enrichment score for the GO pathways found significantly enriched in the whole crypt RNA-seq. The scatter plots show the correlation between enrichment scores found in Lgr5^hi intestinal stem cells (Y-axis) versus whole crypts (X axis) RNA-seq. Percentage indicates the pathways found statistically enriched both in Lgr5^hi intestinal stem cells and whole crypts. d Hierarchical clustering and heatmap of the gene overlap correlation of the top 25 GO pathways found enriched in the Lgr5^hi intestinal stem cells during aging. Red-marked pathways are related to immune system signaling and inflammation. e The bar chart shows the percentage of MHCII⁺ cells from Olfm4⁺ intestinal stem cells in the indicated groups. n = 3 mice per group were analyzed. Error bars represent the SD. P value was calculated by two-sided Welch’s t test. f, g Box plot of geneset enrichment analysis of the indicated gene datasets in the Lgr5^hi intestinal stem cells purified from female (F) and male (M) mice, young (Y) and old (O) mice. n = 3 mice per group were analyzed. P value was calculated by two-sided Wilcoxon paired test. ns  not significant.