Fig. 5: IL-1-dependent activation of perivascular bone marrow stromal cells enhance Vwf + HSC activity in response to platelet depletion. | Nature Communications

Fig. 5: IL-1-dependent activation of perivascular bone marrow stromal cells enhance Vwf + HSC activity in response to platelet depletion.

From: Perivascular niche cells sense thrombocytopenia and activate hematopoietic stem cells in an IL-1 dependent manner

Fig. 5: IL-1-dependent activation of perivascular bone marrow stromal cells enhance Vwf + HSC activity in response to platelet depletion.The alternative text for this image may have been generated using AI.

(Related to Supplementary Fig. 6). a–c Analysis of mice with conditional deletion of Il1r1 in Lepr+ perivascular cells (Il1r1FL/FL Lepr-CreTg/+) mice after platelet depletion. Controls include Il1r1FL/+ Lepr-CreTg/+, Il1r1+/+ Lepr-CreTg/+, Il1r1FL/FL Lepr-CreTg/+ (IgG only) and Lepr-Cre+/+ mice. a Cell cycle analysis of Vwf+ and VWF– HSCs 1 day post platelet depletion. Data represent mean ± SD frequencies of 3 (control-IgG), 5 (control-GPIbα) and 5 (Il1r1FL/FL Lepr-CreTg/+-GPIbα) mice from 3 independent experiments. b Cell cycle analysis of Vwf+ and VWF– HSCs from mice 1 day post intravenous administration of 50ug/Kg of IL-1β. Data are mean ± SD of 3 (control-IgG), 6 (control-GPIbα) and 4 (Il1r1FL/FL Lepr-CreTg/+-GPIbα) mice from 3 independent experiments. c Peripheral blood analysis of platelet recovery at the indicated time points post platelet depletion. Mean ± SD platelet numbers from 6 (control) and 11 (Il1r1FL/FL Lepr-CreTg/+) mice in 3 independent experiments. d–g Differential gene expression analysis in Vwf+ and Vwf– HSCs isolated from wild type mice 1 day post platelet depletion (GPIbα treatment). d Volcano plots of genes differentially expressed in Vwf+ and Vwf– HSCs. Red dots indicate genes with significant expression differences (adjusted p value (q)<0.05). e Venn diagram showing number of differentially expressed genes in Vwf+ and Vwf– HSCs post platelet depletion and between these HSC subsets in homeostasis (IgG). f GO terms analysis of biological processes/pathways up-regulated in Vwf+ HSCs after platelet depletion. g Expression (FPKM) of genes associated with Activin/BMP signaling in Vwf+ and Vwf– HSCs from Wt mice in homeostasis and 1 day post platelet depletion. All data (d–g) represent mean ± SD FPKM data of 3 biological replicates per genotype and condition. *p < 0.05; **p < 0.01; ***p < 0.001; ns non-significant (p > 0.05); using 2-way ANOVA with Tukey’s multiple comparisons (a, b), 2-way ANOVA with Sidak’s multiple comparisons (c) and two-sided t-test (g). See also Supplementary Fig. 5.

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