Fig. 3: OPC-induced pyroptosis improved CSps cellular bioactivities and paracrine effects. | Nature Communications

Fig. 3: OPC-induced pyroptosis improved CSps cellular bioactivities and paracrine effects.

From: Mechanically induced pyroptosis enhances cardiosphere oxidative stress resistance and metabolism for myocardial infarction therapy

Fig. 3

a Representative image of caspase-1 immunofluorescence staining results of each group. b The mRNA transcription levels of caspase-1 and IL-1β (n = 5 biologically independent samples). c The protein expression levels of caspase-1 and cl-caspase-1 tested by western blot. d The concentration of IL-1β in the cell supernatant after 3 days of cultivation of each group (n = 5 biologically independent samples). e Representative TEM images of the cell ultrastructure in CSps, and 3 biologically independent samples were observed. N: nucleus, yellow arrows indicate mitochondria, yellow asterisks (*) indicate endoplasmic reticulum, and yellow triangles (△) indicate microvesicles. f Annexin/PI analysis results of the cell survival rate (n = 3 biologically independent samples). g Proliferation assay of the CDCs isolated from the PS-CSps, ULA-CSps, and OPC-CSps (n = 5 biologically independent samples). h The mRNA transcription levels of Oct4, Nanog, and Sox2 (n = 5 biologically independent samples). i The mRNA transcription levels of VEGF, bFGF, HGF, and IGF-1 (n = 5 biologically independent samples). All data are shown as the mean ± SD, *P < 0.05 vs. PS, #P < 0.05 vs. ULA, one-way ANOVA (d, f) or two-way ANOVA (b, g, h, i).

Back to article page