Fig. 7: The reduction in cardiac inflammation, apoptosis, and hypertrophy after OPC-CSps transplantation. | Nature Communications

Fig. 7: The reduction in cardiac inflammation, apoptosis, and hypertrophy after OPC-CSps transplantation.

From: Mechanically induced pyroptosis enhances cardiosphere oxidative stress resistance and metabolism for myocardial infarction therapy

Fig. 7

a Representative images of Masson trichrome staining 12 weeks following MI, and yellow arrows mark the blood vessels in the border of the infarct area. (n = 8 rats). b Representative images of CD68+ macrophages in the peri-infarct zone 2 weeks following MI, α-SMA+ CD31+ vessels in the infarct zone 12 weeks following MI, and TUNEL+ cells in the peri-infarct zone 12 weeks following MI (n = 15 images from 3 rats). Spilt channels of the α-SMA and CD31 staining results are shown in Supplementary Fig. 4 (n = 15 images from 3 rats). c Quantitative results of the infarct area (n = 8 rats). d The percentage of viable myocardium at the infarct area (n = 8 rats). e Quantitative results of infarct wall thickness (n = 8 rats). f The quantitative results of CD68+ macrophages per HPF assessed by ImageJ software V1.8.0.112. HPF high-power field (n = 15 images from 3 rats). g The quantitative results of vessel density of each group (n = 15 images from 8 rats). h The quantitative results of the TUNEL+ rate assessed by ImageJ software V1.8.0.112 (n = 15 images from 8 rats). i Representative images of WGA staining of heart tissues shown in different regions at 12 weeks. The cardiomyocyte membrane was stained with WGA (green), cardiomyocytes were identified by staining for cTnT (red), and DAPI showed nuclei. Quantitative analysis of cardiomyocyte cross-sectional area from j the left ventricle, k the border zone, and l the remote zone (n = 15 images from 8 rats). Each data point is represented as the mean ± SD, *P < 0.05 vs. sham, #P < 0.05 vs. vehicle, Two-sided student’s t test (c, d) or one-way ANOVA (e, f, g, h, j, k, l).

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